During untreated primary HIV infection, people have exceptionally high
viral loads and are extremely infectious. This has been cited as a limiting
factor in test-and-treat programmes since, if a high proportion of infections
are transmitted by people in primary infection, this places a limit on the
degree to which diagnosing people with HIV and treating them promptly can
reduce onward transmission. People in primary infection (within the first six
months) are less likely to be diagnosed, at least until after their period of
peak infectiousness has passed. Moreover, HIV-antibody tests will not usually
detect infection in the first few weeks after exposure, and even p24 or RNA
tests are not reliable in the first two to three weeks.
However, primary
infection only lasts a few weeks or months, so there are fewer opportunities
for transmission than during many years of chronic infection. The opportunity
for transmission is therefore lower compared with untreated chronic HIV
infection, which can last for years or even decades.
Frequency of partner change will also have a major impact on
the role of primary infection: the more frequently partners change within a
high-risk population, the greater the potential for early onward transmission.
As a result, there is currently some disagreement regarding
the impact of untreated primary
(also called acute) HIV infection versus untreated chronic HIV infection on
infectiousness and sexual transmission, and their relative contributions to
onward transmission, with estimates of transmission following primary infection
ranging from a low of 9% to a high of almost 50%. The proportion probably
varies with the characteristics of the population studied, such as the rate of
partner change, the proportion of HIV transmitted in stable couples and the proportion
transmitted during casual sex.
A prospective study in heterosexual couples in Rakai,
Uganda,1 where one partner was
HIV-positive at the start of the study, and one partner HIV-negative, further
established that the likelihood of heterosexual HIV transmission is highest in
the first two and a half months following initial infection with HIV, and that
this correlates with higher viral load levels in early HIV infection. They
estimated that 43% of transmissions in the study occurred within ten weeks of
the index partner’s infection.
The investigators also estimated that primary HIV infection
increased the risk of HIV transmission per sexual act more than seven-fold
compared to the risk of HIV transmission per sexual act during the chronic (and
longest) period of untreated HIV infection. Further analysis of these data2 estimated that relative to
chronic infection, infectiousness during primary infection was actually
enhanced 26-fold (and 3.6-fold during late/end-stage infection).
However, taking into account primary infection’s relatively
short duration, the study estimated that 14% of onward transmission occurred
during primary infection (compared with 46% during the chronic stage and 40%
during end/late-stage HIV disease).
Similarly, a 2007 US mathematical modelling study3 estimated that fewer than
9% of all new sexually transmitted HIV infections originated in people with
untreated primary HIV infection, compared with 48% of new infections resulting
from sexual contact with people with untreated chronic HIV infection.
Although it agreed with previous studies that individuals
with primary HIV infection have exceptionally high viral loads, and estimated
that they are 16 times more infectious than during chronic infection, because
the period for which they are highly infectious is relatively short, typically
no more than 49 days, individuals with acute HIV infection only represent 0.5%
of all HIV-infected individuals in the US.
Studies in populations that include a higher proportion of
gay men and other MSM (with potentially much more frequent partner change than
heterosexual couples in Africa, even those with concurrent relationships), have
come to slightly different conclusions, partially due to their more liberal
interpretation of primary infection.
A 2007 study from Quebec, Canada,4 used phylogenetic analysis
of blood samples in Quebec’s
genotypic-resistance database. It estimated that almost half of all sexually
transmitted HIV infections were attributed to primary or early infection.
However, in this study, although some onward transmission took place within a
month of infection, the average time between infection and onward transmission
was 15 months.
The study concluded that whilst primary/early HIV infection
represented just 10% of the total sequenced samples in the genotypic-resistance
database, it accounted for 49% of all onward transmission events. In contrast,
the study found that treatment-naive and treatment-experienced chronically
infected individuals accounted for 15% and 12% of onward transmission,
respectively, with the other 25% uncertain.
A similar phylogenetic study, focused primarily on gay men
attending London’s
largest HIV clinic,5
estimates that around 25% of onward HIV transmission took place within six
months of infection.
A third study from Switzerland, using the
same methods, concluded that about 30% of new infections showed signs of being
transmitted in early infection.6
Perhaps the most intriguing study on the relative impact of
primary versus chronic infection comes from investigators from Imperial College,
London, which
used mathematical modelling to estimate the impact of untreated HIV infection
on transmission.7
The main aim of the study was to quantify the relationship
between viral load and infectiousness and to estimate its epidemiological impact.
The investigators examined the link between viral load and transmission from a
Zambian study of serodiscordant heterosexual couples and found that the periods
of highest viral load (during primary infection and again during late-stage HIV
disease) did not actually have the highest transmission potential, because
these lasted relatively short periods of time. Instead, they found that the
viral load with the highest transmission potential (of a hypothetical average
of 1.5 infections per person per lifespan) was found to be during chronic
infection.
The study concluded that the 'ideal' viral load for HIV in
its 'quest' for continued survival was 33,113 (4.52 log10)
copies/ml. At this viral load, someone could live for around ten years without
becoming ill and still feel well enough to have sex, providing HIV with the
longest transmission potential. The investigators hypothesise that HIV may have
actually evolved so that average viral loads during chronic infection are
finely balanced between being the optimal for HIV transmission and the optimal
for human survival.